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mouse immortalized leydig cell lines (ATCC)

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ATCC mouse immortalized leydig cell lines

(A) Representative aspect of cells after 24 h of exposure to ethylene dimethanesulfonate (EDS) of rat R2C and mouse MA-10 <t>Leydig</t> cells. Note that at 5 mM, the cell confluency and morphological characteristic seem qualitatively impaired (red arrows; insert), indicating the cytotoxicity caused by EDS. (B) Cell count of R2C and MA-10 cells after 4 and 24 h of EDS exposure at concentrations of 1 or 2 mM (n = 4, each in duplicate). Values are expressed as median (interquartile ranges). Kruskal-Wallis followed by Dunn’s test (p > 0.05).

Mouse Immortalized Leydig Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+tumor+leydig+cell+line+ma+10/pmc10792691-46-2-8?v=ATCC
Average 94 stars, based on 22 article reviews

mouse immortalized leydig cell lines - by Bioz Stars, 2026-07

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1) Product Images from "Ethylene dimethanesulfonate effects on gene promoter activities related to the endocrine function of immortalized Leydig cell lines R2C and MA-10"

Article Title: Ethylene dimethanesulfonate effects on gene promoter activities related to the endocrine function of immortalized Leydig cell lines R2C and MA-10

Journal: Current Research in Toxicology

doi: 10.1016/j.crtox.2023.100147

(A) Representative aspect of cells after 24 h of exposure to ethylene dimethanesulfonate (EDS) of rat R2C and mouse MA-10 Leydig cells. Note that at 5 mM, the cell confluency and morphological characteristic seem qualitatively impaired (red arrows; insert), indicating the cytotoxicity caused by EDS. (B) Cell count of R2C and MA-10 cells after 4 and 24 h of EDS exposure at concentrations of 1 or 2 mM (n = 4, each in duplicate). Values are expressed as median (interquartile ranges). Kruskal-Wallis followed by Dunn’s test (p > 0.05).

Figure Legend Snippet: (A) Representative aspect of cells after 24 h of exposure to ethylene dimethanesulfonate (EDS) of rat R2C and mouse MA-10 Leydig cells. Note that at 5 mM, the cell confluency and morphological characteristic seem qualitatively impaired (red arrows; insert), indicating the cytotoxicity caused by EDS. (B) Cell count of R2C and MA-10 cells after 4 and 24 h of EDS exposure at concentrations of 1 or 2 mM (n = 4, each in duplicate). Values are expressed as median (interquartile ranges). Kruskal-Wallis followed by Dunn’s test (p > 0.05).

Techniques Used: Cell Counting

A dose–response study with reporter luciferase assays to determine the effects of EDS on (A) Star , (B) Cyp17a1 , (C) Insl3 , and (D) Gsta3 promoter activity. Rat R2C (n = 4, each in triplicate) and mouse MA-10 (n = 5, each in triplicate) Leydig cells were transfected with different promoter constructs and treated for 24 h with increasing concentrations of EDS as indicated. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. ANOVA followed by Dunnett’s test (*p < 0.05 compared to the control group).

Figure Legend Snippet: A dose–response study with reporter luciferase assays to determine the effects of EDS on (A) Star , (B) Cyp17a1 , (C) Insl3 , and (D) Gsta3 promoter activity. Rat R2C (n = 4, each in triplicate) and mouse MA-10 (n = 5, each in triplicate) Leydig cells were transfected with different promoter constructs and treated for 24 h with increasing concentrations of EDS as indicated. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. ANOVA followed by Dunnett’s test (*p < 0.05 compared to the control group).

Techniques Used: Luciferase, Activity Assay, Transfection, Construct, Control

EDS effects on the activity of the Insl3 and Gsta3 gene promoter after 4 and 24 h of exposure to EDS of rat R2C (1 mM of EDS, n = 4–5, each in triplicate) and mouse MA-10 (2 mM of EDS, n = 5, each in triplicate) Leydig cells. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. Welch’s t -test (*p < 0.05).

Figure Legend Snippet: EDS effects on the activity of the Insl3 and Gsta3 gene promoter after 4 and 24 h of exposure to EDS of rat R2C (1 mM of EDS, n = 4–5, each in triplicate) and mouse MA-10 (2 mM of EDS, n = 5, each in triplicate) Leydig cells. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. Welch’s t -test (*p < 0.05).

Techniques Used: Activity Assay, Luciferase

EDS effects on Star promoter activity after 4 h of exposure to EDS of rat R2C (1 mM of EDS, n = 5, each in triplicate) and mouse MA-10 (2 mM of EDS, n = 5, each in triplicate) Leydig cells. EDS effects on Star promoter activity after 24 h of exposure to EDS of rat R2C (1 mM of EDS, n = 3, each in triplicate) and mouse MA-10 (2 mM of EDS, n = 5, each in triplicate) Leydig cells, DC3 granulosa cells (1 mM of EDS, n = 5, each in triplicate), and MSC-1 Sertoli cells (2 mM of EDS, n = 5, each in triplicate). EDS effects were assessed in basal and stimulated (0.5 mM 8Br-cAMP) conditions in R2C Leydig cells. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. Welch’s t -test (*p < 0.05).

Figure Legend Snippet: EDS effects on Star promoter activity after 4 h of exposure to EDS of rat R2C (1 mM of EDS, n = 5, each in triplicate) and mouse MA-10 (2 mM of EDS, n = 5, each in triplicate) Leydig cells. EDS effects on Star promoter activity after 24 h of exposure to EDS of rat R2C (1 mM of EDS, n = 3, each in triplicate) and mouse MA-10 (2 mM of EDS, n = 5, each in triplicate) Leydig cells, DC3 granulosa cells (1 mM of EDS, n = 5, each in triplicate), and MSC-1 Sertoli cells (2 mM of EDS, n = 5, each in triplicate). EDS effects were assessed in basal and stimulated (0.5 mM 8Br-cAMP) conditions in R2C Leydig cells. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. Welch’s t -test (*p < 0.05).

Techniques Used: Activity Assay, Luciferase

The EDS-responsive region is located between −400 and −195 bp of the Star promoter. Progressive 5′ deletion constructs of Star gene promoter were transfected in rat R2C Leydig cells and treated with 1 mM EDS for 24 h (n = 3–5, each in triplicate) in the absence or presence of 0.5 mM 8Br-cAMP for 4 h. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. Welch’s t -test (*p < 0.05).

Figure Legend Snippet: The EDS-responsive region is located between −400 and −195 bp of the Star promoter. Progressive 5′ deletion constructs of Star gene promoter were transfected in rat R2C Leydig cells and treated with 1 mM EDS for 24 h (n = 3–5, each in triplicate) in the absence or presence of 0.5 mM 8Br-cAMP for 4 h. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. Welch’s t -test (*p < 0.05).

Techniques Used: Construct, Transfection, Luciferase

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Journal: Current Research in Toxicology

Article Title: Ethylene dimethanesulfonate effects on gene promoter activities related to the endocrine function of immortalized Leydig cell lines R2C and MA-10

doi: 10.1016/j.crtox.2023.100147

Figure Lengend Snippet: (A) Representative aspect of cells after 24 h of exposure to ethylene dimethanesulfonate (EDS) of rat R2C and mouse MA-10 Leydig cells. Note that at 5 mM, the cell confluency and morphological characteristic seem qualitatively impaired (red arrows; insert), indicating the cytotoxicity caused by EDS. (B) Cell count of R2C and MA-10 cells after 4 and 24 h of EDS exposure at concentrations of 1 or 2 mM (n = 4, each in duplicate). Values are expressed as median (interquartile ranges). Kruskal-Wallis followed by Dunn’s test (p > 0.05).

Article Snippet: Rat and mouse immortalized Leydig cell lines, R2C (ATCC, Manassas, Virginia, USA) and MA-10 (ATCC, Manassas, Virginia, USA), respectively, were used for this study from passages 40 to 52 (R2C) or 08 to 31 (MA-10).

Techniques: Cell Counting

Journal: Current Research in Toxicology

Article Title: Ethylene dimethanesulfonate effects on gene promoter activities related to the endocrine function of immortalized Leydig cell lines R2C and MA-10

doi: 10.1016/j.crtox.2023.100147

Figure Lengend Snippet: A dose–response study with reporter luciferase assays to determine the effects of EDS on (A) Star , (B) Cyp17a1 , (C) Insl3 , and (D) Gsta3 promoter activity. Rat R2C (n = 4, each in triplicate) and mouse MA-10 (n = 5, each in triplicate) Leydig cells were transfected with different promoter constructs and treated for 24 h with increasing concentrations of EDS as indicated. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. ANOVA followed by Dunnett’s test (*p < 0.05 compared to the control group).

Techniques: Luciferase, Activity Assay, Transfection, Construct, Control

Journal: Current Research in Toxicology

Article Title: Ethylene dimethanesulfonate effects on gene promoter activities related to the endocrine function of immortalized Leydig cell lines R2C and MA-10

doi: 10.1016/j.crtox.2023.100147

Figure Lengend Snippet: EDS effects on the activity of the Insl3 and Gsta3 gene promoter after 4 and 24 h of exposure to EDS of rat R2C (1 mM of EDS, n = 4–5, each in triplicate) and mouse MA-10 (2 mM of EDS, n = 5, each in triplicate) Leydig cells. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. Welch’s t -test (*p < 0.05).

Techniques: Activity Assay, Luciferase

Journal: Current Research in Toxicology

Article Title: Ethylene dimethanesulfonate effects on gene promoter activities related to the endocrine function of immortalized Leydig cell lines R2C and MA-10

doi: 10.1016/j.crtox.2023.100147

Figure Lengend Snippet: EDS effects on Star promoter activity after 4 h of exposure to EDS of rat R2C (1 mM of EDS, n = 5, each in triplicate) and mouse MA-10 (2 mM of EDS, n = 5, each in triplicate) Leydig cells. EDS effects on Star promoter activity after 24 h of exposure to EDS of rat R2C (1 mM of EDS, n = 3, each in triplicate) and mouse MA-10 (2 mM of EDS, n = 5, each in triplicate) Leydig cells, DC3 granulosa cells (1 mM of EDS, n = 5, each in triplicate), and MSC-1 Sertoli cells (2 mM of EDS, n = 5, each in triplicate). EDS effects were assessed in basal and stimulated (0.5 mM 8Br-cAMP) conditions in R2C Leydig cells. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. Welch’s t -test (*p < 0.05).

Techniques: Activity Assay, Luciferase

Journal: Current Research in Toxicology

Article Title: Ethylene dimethanesulfonate effects on gene promoter activities related to the endocrine function of immortalized Leydig cell lines R2C and MA-10

doi: 10.1016/j.crtox.2023.100147

Figure Lengend Snippet: The EDS-responsive region is located between −400 and −195 bp of the Star promoter. Progressive 5′ deletion constructs of Star gene promoter were transfected in rat R2C Leydig cells and treated with 1 mM EDS for 24 h (n = 3–5, each in triplicate) in the absence or presence of 0.5 mM 8Br-cAMP for 4 h. Luciferase reporter (Luc). Values are expressed as mean ± S.E.M. Welch’s t -test (*p < 0.05).

Techniques: Construct, Transfection, Luciferase

FIG. 4. Effect of INSL3 on mouse Leydig TM3 cell proliferation. A) Mouse TM3 cell proliferation after 48 and 72 h of incubation with different amounts of INSL3 peptide. B) TM3 cell proliferation after incubation with 100 ng/ml of INSL3, 109 M of R1881, INSL3 plus R1881, or vehicle (control) in serum-free medium for 24, 48, and 72 h. Cell proliferation was measured by CellTiter 96 Aqueous One Solution proliferation assay kit (Promega). Each column represents the mean 6 SEM of two independent experiments in quintuplicate. Different treatments were compared to the appropriate vehicle controls, *P , 0.05; **P , 0.01; ***P , 0.001. C) Relative expression analysis of Rxfp2 in mLTC-1 cells, adult testis, and TM3 cells. The Hmbs expression was used as a loading control.

Journal: Biology of reproduction

Article Title: Developmental expression and gene regulation of insulin-like 3 receptor RXFP2 in mouse male reproductive organs.

doi: 10.1095/biolreprod.107.060442

Figure Lengend Snippet: FIG. 4. Effect of INSL3 on mouse Leydig TM3 cell proliferation. A) Mouse TM3 cell proliferation after 48 and 72 h of incubation with different amounts of INSL3 peptide. B) TM3 cell proliferation after incubation with 100 ng/ml of INSL3, 109 M of R1881, INSL3 plus R1881, or vehicle (control) in serum-free medium for 24, 48, and 72 h. Cell proliferation was measured by CellTiter 96 Aqueous One Solution proliferation assay kit (Promega). Each column represents the mean 6 SEM of two independent experiments in quintuplicate. Different treatments were compared to the appropriate vehicle controls, *P , 0.05; **P , 0.01; ***P , 0.001. C) Relative expression analysis of Rxfp2 in mLTC-1 cells, adult testis, and TM3 cells. The Hmbs expression was used as a loading control.

Article Snippet: The effect of INSL3 on cell proliferation was assessed using primary gubernacular cell cultures and two mouse Leydig cell lines, mouse Leydig tumor cells (mLTC-1; American Type Culture Collection no. CRL-2065) and TM3 cell line (American Type Culture Collection no. CRL-1714), established from the prenatal mouse testis.

Techniques: Incubation, Control, Proliferation Assay, Expressing

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